A concise guide to cDNA microarray analysis.

Concise Guide to cDNA Microarray Analysis – II

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling, and hybridization, and we have developed strategies for data no...

cDNA Microarray Data Normalization

Identification of specific gene expression after exposure to low dose ionizing radiation revealed through integrative analysis of cDNA microarray data and the interactome

Background: Accumulating reports suggest that the biological effects of low- and high- dose ionizing radiation (LDIR and HDIR) are qualitatively different and might cause different effects in human skin. Materials and Methods: To better understand the potential risks of LDIR, we analyzed three cDNA microarray datasets from the Gene Expression Omnibus database. Results: A pathway analysis showed...

Using Gene Expression Profiling and Artificial Neural Networks for Patients with Neuroblastoma

EP 1683 862 A1 T 2006 Khan et al., “Classification and diagnostic prediction of cancers using gene expression profiling and artificial neural networks', Nature Medicine, 7(6):673-679 (2001). Barrett et al., “Microarrays: the use of oligonucleotides and cDNA for the analysis of gene expression'. Drug Discovery Today, 8(3): 134-141 (2003). Berwanger et al., “Loss of a FYN-regulated Differentiatio...

پاسخ متفاوت سلولهای قلبی به اسیدهای چرب اشباع و غیر اشباع

Introduction & Objective: The link between dietary fat and coronary heart disease has attracted much attention since the effect of long?chain fatty acids (LCFA) on gene transcription has been established, which in part, these effects can be explained by the regulation of gene transcription. In this study, the P19CL6 cardiac cell?line was targeted for the investigation of (i) the effects of long...